Volume 12, Issue 45 (11-2021)
NCMBJ 2021, 12(45): 51-61 |
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Kouroshnia A, Zeinali S, Irani S, Sadeghi A. Evaluation of the cytotoxicity of antagonist actinomycetes on colorectal cancer cell line. NCMBJ 2021; 12 (45) :51-61
URL: http://ncmbjpiau.ir/article-1-1446-en.html
URL: http://ncmbjpiau.ir/article-1-1446-en.html
Department of Biology, Islamic Azad University Science and Research Brach, Tehran, Iran
Abstract: (2237 Views)
Aim and Background: Actinomycetes are a good resource to discover new drugs based on their abundant secondary metabolites. Because both fungal and human cells are eukaryotes, the metabolites of fungal antagonist actinomycetes may also inhibit the growth of cancer cells.
Materials and Methods: The antagonistic activity of 24 actinomycete strains that inhibited the growth of cells of a plant pathogen, Phytophthora capsici, was investigated against 4 other plant pathogens. The siderophores production of two selected strains that showed the highest antagonistic activity against pathogens was evaluated. The cytotoxicity effects of two strains on the SW480 cell line was measured using MTT assay in vitro and the IC50 percentage was determined.
Results: Only two selected strains had 30 and 54% inhibitory effect against Phytophthora drechsleri, respectively. The strain 408 had no inhibitory effect against Pythium ultimum, while the strain 6010 completely prevented Pythium ultimum growth. The strain 6010, unlike the strain 408, controlled the growth of Rhizoctonia solani and Fusarium oxysporum pathogens about 70%. Only the strain 408 was able to produce siderophore. The significant effect of the treatment on colorectal cancer cells was observed at 2.47 and 2.71% (v/v) concentrations for both 408 and 6010 strains, respectively.
Conclusion: The results of this study confirmed our hypothesis that secondary metabolites of antagonistic actinomycetes can lead to cancer cells death. The use of fungal or oomycete cells is introduced as an easy, safe, and inexpensive method for screening actinomycetes that are candidates for the discovery of new anticancer drugs.
Materials and Methods: The antagonistic activity of 24 actinomycete strains that inhibited the growth of cells of a plant pathogen, Phytophthora capsici, was investigated against 4 other plant pathogens. The siderophores production of two selected strains that showed the highest antagonistic activity against pathogens was evaluated. The cytotoxicity effects of two strains on the SW480 cell line was measured using MTT assay in vitro and the IC50 percentage was determined.
Results: Only two selected strains had 30 and 54% inhibitory effect against Phytophthora drechsleri, respectively. The strain 408 had no inhibitory effect against Pythium ultimum, while the strain 6010 completely prevented Pythium ultimum growth. The strain 6010, unlike the strain 408, controlled the growth of Rhizoctonia solani and Fusarium oxysporum pathogens about 70%. Only the strain 408 was able to produce siderophore. The significant effect of the treatment on colorectal cancer cells was observed at 2.47 and 2.71% (v/v) concentrations for both 408 and 6010 strains, respectively.
Conclusion: The results of this study confirmed our hypothesis that secondary metabolites of antagonistic actinomycetes can lead to cancer cells death. The use of fungal or oomycete cells is introduced as an easy, safe, and inexpensive method for screening actinomycetes that are candidates for the discovery of new anticancer drugs.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2021/07/10 | Accepted: 2021/10/28 | Published: 2021/12/22
Received: 2021/07/10 | Accepted: 2021/10/28 | Published: 2021/12/22
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