Volume 4, Issue 16 (12-2014)
NCMBJ 2014, 4(16): 29-35 |
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Fooladvand S, Yari R, Nanekarani S. Study of DGAT-1 gene polymorphism in Mehraban-sheep by PCR-RFLP technique. NCMBJ 2014; 4 (16) :29-35
URL: http://ncmbjpiau.ir/article-1-412-en.html
URL: http://ncmbjpiau.ir/article-1-412-en.html
Azad University, Boroujerd , fouladvand.s@gmail.com
Abstract: (9544 Views)
Aim and Background: The aim of this study to compare the coding area of the exon 17 DGAT1 gene polymorphism in Mehraban sheep.
Materials and Methods: DNA was extracted using Kit method, 120 samples of Mehraban sheep were obtained, and also polymerase chain reaction was performed to amplify 309 bp of exon 17 DGAT1 gene using a specific primer. In RFLP method, PCR products using AluI enzyme digestion were digested and the three genotypes TT, TC and CC were found.
Conclusion: Mehraban sheep in two alleles T and C respectively, with frequency of 0.37 and 0.63 were observed. Also the population was not found to follow Hardy-Weinberg equilibrium.
Materials and Methods: DNA was extracted using Kit method, 120 samples of Mehraban sheep were obtained, and also polymerase chain reaction was performed to amplify 309 bp of exon 17 DGAT1 gene using a specific primer. In RFLP method, PCR products using AluI enzyme digestion were digested and the three genotypes TT, TC and CC were found.
Conclusion: Mehraban sheep in two alleles T and C respectively, with frequency of 0.37 and 0.63 were observed. Also the population was not found to follow Hardy-Weinberg equilibrium.
Type of Study: Research Article |
Subject:
Genetics
Received: 2013/11/7 | Accepted: 2014/10/27 | Published: 2014/12/27
Received: 2013/11/7 | Accepted: 2014/10/27 | Published: 2014/12/27
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