Aim and Background. Stem cells have self-renewal potential and differentiating ability into adult somatic cell types. In this study the mesenchymal stem cells of human umbilical cord Wharton’s jelly were isolated. After proliferation, they were differentiated into blood cells via treating them by rat lung tissue extract which is rich in G-CSF, M-CSF,GM-CSF,IL-6 cytokines essential for various steps of differentiation.

 Materials and Methods. Human umbilical cord Wharton’s jelly tissue was cut into 0.5 cm, and then cultured in T25 flask. After second passage, mesenchymal stem cells were treated with continuous lung tissue extract for differentiation. The cells cultured in 1:3, 1:1and 3:1 experimental volume groups of DMEM: extract ratio.

 Results. Blood cells were observed at 1:3, 3:1 and 1:1 DMEM: extract experimental groups on the seventh day. Mesenchymal stem cells were confirmed by flow cytometric analysis (CD90,CD105). Positive cells were considered as mesenchymal stem cell in human Wharton’s jelly. Microscopic analysis of cells after Giemsa staining verified the presence of blood cells.

 Conclusion. Human Wharton’s jelly mesenchymal stem cells were isolated by tissue culture methods . Also mouse cytokines could selectively induce human stem cells to adult human blood cells.